FIGURE 3.

3,3′-diaminobenzidine (DAB) immunohistochemistry of MFSD14A in the mouse brain. Seventy micrometers of coronal sections from adult mice brains were stained for MFSD14A. Schematic brain atlas description was modified from the 2015 Allen Institute for Brain Science. Allen Mouse Brain Atlas available from http://mouse.brain-map.org (Lein et al., 2007). (A) Brain section from bregma 0.26 mm with close ups from areas of the cortex and striatum show abundant staining of cell bodies. (B) Brain section from bregma -1.94 mm with close ups from hippocampus, and from around the third ventricle, including areas of the hypothalamus. Abundant staining was found in all areas of the cortex, the piriform hippocampus, including dentate gyrus, and areas around both the dorsal third ventricle and the third ventricle. (C) Brain section from bregma -5.80 mm, with close ups of heavily stained cells in the third cerebellar lobule and large cells in intermedial reticular nucleus. The scale bars for the adjacent magnifications represent 100 μm.