FIGURE 4.

DAB immunohistochemistry of MFSD14B in the mouse brain. Seventy micrometers coronal sections from adult mice brains were stained with anti-HIATL1 antibody (SAB2107506, Sigma-Aldrich). Schematic brain atlas description was modified from the 2015 Allen Institute for Brain Science. Allen Mouse Brain Atlas available from http://mouse.brain-map.org (Lein et al., 2007). (A) Brain section from bregma 0.26 mm with close ups from areas of the cortex and striatum show staining of scattered cells. Both cell bodies as well as projections are stained. (B) Brain section from bregma -1.94 mm with close ups from hippocampus, and from around the third ventricle, including areas of the hypothalamus. Stained cells were found scattered in all areas of the cortex, hippocampus, and thalamus. Around both the third ventricle larger cells with clear projections were found. (C) Brain section from bregma -5.80 mm, with close ups of cerebellar lobule and large heavily stained cells in the facial nucleus. The scale bars for the adjacent magnifications represent 100 μm.