Figure 2.

CB1R activity is necessary for dendritic development. (A) 6-DIV hippocampal neurons treated with CB1R antagonists, and/or agonist or treated with the MAGL inhibitor UCM03025. Drugs were first added after plating and treatment was repeated every other day. After 6 days in culture, neurons were stained with MAP2 antibody to detect the somatodendritic domain and DAPI for nuclei. Scale bar = 100 μm. (B) Bar plots representing the mean ± SEM of total dendritic length. Data were acquired from three independent experiments (30 neurons/experimental condition in each experiment). Kruskal-Wallis, Dunn’s multiple comparisons test. Adjusted p values: ****p < 0.0001; ns, non-significant.