Figure 3.

CB1R inhibition decreases ankyrinG expression at the developing AIS. (A,B) 6-DIV hippocampal neurons treated with 2-AG or UCM-03025 (A), or 2-AG in combination with AM251 (B). AIS domain stained for ankyrinG is included in a box and its magnification shown below (A) or in the right (B) for every image. Drugs were first added after cell plating and treatment was repeated every other day. After 6 days in culture, neurons were fixed and stained for MAP2 (red) to visualize somatodendritic compartment, and ankyrinG (AnkG, green) to visualize the AIS. All images were acquired using the same fluorescence parameters. Scale bar = 100 μm. (C) Bar plots representing the mean ± SEM of normalized AnkyrinG total fluorescence intensity along the AIS. Data were acquired from three independent experiments (30 neurons/experimental condition in each experiment). ANOVA, Bonferroni multiple comparisons test. Adjusted p values: **p < 0.01; *p < 0.05; ns, non-significant; a.u., arbitrary units.