Figure 5.

Glial type-2 cannabinoid receptors (CB2Rs) modulation of AIS. (A,B) 10-DIV hippocampal neurons treated daily with the CB2R antagonist AM630 from 7 to 10 days. Neurons were grown in the presence of a glial cell layer until 7 days (co-culture). Then, one group was treated in the presence of glial cells (A) and the second group was transferred to other plates containing only conditioned medium (B). Neurons were stained with the somatodendritic marker MAP2 (red) and ankyrinG (green). Scale bar = 100 μm. (C,D) Graphs representing normalized ankyrinG fluorescence intensity profile along the AIS in control or AM630-treated neurons in the presence (C) or absence (D) of glial cells. (E) Graph representing the normalized total ankyrinG fluorescence intensity at the AIS of neurons represented in (C,D). Data were acquired from three independent experiments (30 neurons/experimental condition in each experiment). Mann-Whitney Rank test. *p < 0.05.