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Principle of Allele-specific primer extension (ASPE) (red and green line = anti-TAGs; green star = fluorescent reporter). Allele-specific detection probes, differing in one nucleotide on the polymorphic side, hybridize to amplified target sequence. After addition of DNA polymerase and dNTPs (one of which is fluorescently labeled), molecules are extended according to complementarity. Products are captured by anti-TAGs on the specific microsphere set.
