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Macrophage migration inhibitory factor (MIF) secretion by newborn and adult leukocyte subsets. Neutrophils, monocytes, B cells, and T cells from healthy term newborns (white bars) and adult volunteers (black bars) were cultured in RPMI medium supplemented with 10% FCS. MIF levels were measured by enzyme-linked immunosorbent assay in supernatant collected at 24 and 48 h. Data represent means ± SEM of five to nine independent experiments performed in triplicates (*P < 0.05).
