Fig. 2.

Detection of S. aureus proteins during biofilm formation on PS. Biofilm mass formed on PS by 9 clinical isolates and reference strain NCTC 8325 (a). The levels of remaining non-bound IgG directed against specific proteins in PHG after incubation with the biofilms [expressed as mean fluorescence intensity (MFI)], indirectly and inversely reflecting protein presence, are shown for glucosaminidase (b), alpha toxin (c), the non-S. aureus control protein PpmA (d). Please note the difference in range of MFI on the y-axis between Fig b–d. Dashed horizontal lines indicate the average MFI of sterile controls. Symbols and error bars indicate mean and SD of two separate experiments