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Iron (Fe) and zinc (Zn) content in T4 grains and flour of lines expressing OsNAS2 (Nas-BW). Values are the mean of three biological replicates (± SD). Black asterisks above the bars indicate statistically significant values calculated using Student’s T test, in comparison to the control line Bobwhite (BW) (*P < 0.05; **P < 0.01). NTS non-transgenic sibling
