Figure 1.

MLL-AF4 Is Recruited Exclusively to uCpG Regions Bound by Menin

(A) Schematic showing MLL and MLL fusion protein interaction sites.

(B) Schematic showing the MLL-AF4 core complex.

(C) Example ChIP-seq, Bio-CAP-seq, and ATAC-seq tracks in SEM cells.

(D) Venn diagram showing overlap between two biological replicates of MLL(N) ChIP-seq.

(E) Heatmap showing ChIP-seq, Bio-CAP-seq, and ATAC-seq reads at all 4,427 MLL-AF4 binding sites in SEM cells. Scale bar represents tags per base pair (bp) per 10⁷ reads.

(F) Venn diagram showing overlap between MLL-AF4 binding sites and uCpG regions (Bio-CAP-seq and ATAC-seq) in SEM cells.

(G) Heatmap showing MLL(N), AF4(C), and Menin ChIP-seq reads at all MLL-AF4 binding sites in SEM cells. Scale bar as in (E).

(H) Venn diagram showing overlap between MLL-AF4, PAF1, and Menin binding sites in SEM cells.

(I and J) Scatterplot showing a strong correlation (r² = 0.96) between MLL(N) and Menin ChIP-seq signal at all MLL-AF4 peaks (I) in SEM cells and a weak correlation between Menin and CFP1 (r² = 0.27) at all CFP1 peaks (J) in SEM cells.

See also Figure S1.