FIG 1 .

Mutant library screening reveals putative Cst6 kinase. The S. cerevisiae wild type (WT) and mutants were grown in 5% CO₂ to the exponential phase and were either maintained at 5% CO₂ or transferred to air for 60 min. ScNCE103 expression was measured by qRT-PCR and normalized to WT expression under 5% CO₂ (for all data, see Table S2). ScSch9 was the most promising Cst6 kinase candidate. The bars show means ± standard deviations from ≥5 independent experiments. Significance against WT expression under the corresponding condition was calculated with a two-sided t test for unpaired samples and defined as P ≤ 0.05 (*) and P ≤ 0.01 (**).