FIG 4 .

HD5 does not induce dissociation of L2 and the viral genome. (A) Infection of HeLa cells by uncleaved HPV16 L2-FLAG EdU PsV in the presence or absence of 10 µM HD5 in complete medium. Data from three independent experiments are normalized to infection in the absence of inhibitor, ± standard deviation. (B) Images of HeLa cells costained for L2-FLAG and EdU genome at 16 h postinfection in the presence or absence of 10 µM HD5. Individual panels depict signal above threshold for images in the z-stack that are coplanar with the nucleus for L2-FLAG (red) and HPV16 genome (green). In the merged images, the nucleus is blue. Bar, 10 µm. (C to E) Manders coefficient values M2 (genome colocalized with L2) (C), Manders coefficient values M1 (L2 colocalized with genome) (D), and the ratio of the integrated density of the L2-FLAG signal to EdU signal above threshold (E) are plotted as percentages for at least 50 cells each in the absence (-) or presence (+) of HD5. Whiskers are 5 to 95%, the horizontal line is the median, and outliers are depicted as individual points. ****, P < 0.0001.