FIG 5 .

HD5 inhibits trafficking of HPV16 L2 to the trans-Golgi network. (A) Images of HeLa cells costained for TGN46 and L2-FLAG at 16 h postinfection in the presence or absence of HD5 or HD5 Abu. Individual panels depict signal above threshold for images in the z-stack that are coplanar with the nucleus for TGN46 (red) and L2-FLAG (green). In the merged images, the nucleus is blue. Bar, 10 µm. (B) Manders coefficient values M1 (L2 colocalized with TGN46) are plotted as a percentage for 40 to 150 cells for each condition. Whiskers are 5 to 95%, the horizontal line is the median, and outliers are depicted as individual points. ****, P < 0.0001, comparing HD5-treated cells to either untreated or HD5 Abu-treated cells. All other comparisons are not significant.