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. 2017 Jan 17;46(1):120–132. doi: 10.1016/j.immuni.2016.12.011

Figure 1.

Figure 1

Lymphocyte Numbers Exhibit Circadian Oscillations in Lymph Nodes

(A) Lymphocyte oscillations in blood (left panel) and inguinal lymph node (middle and right panels) over 24 hr. Zeitgeber time (ZT, time after light onset) 1 is double-plotted to facilitate viewing; n = 4–49 mice, one-way ANOVA, WBC: white blood cells.

(B) Lymph node oscillations under light-dark (LD), dark-dark (DD) and inverted, dark-light (DL) conditions, normalized to peak times; CT, circadian time in constant darkness conditions; n = 3–15 mice, one-way ANOVA.

(C) Oscillations across multiple lymph nodes, axi: axillary, sup: superficial cervical, ing: inguinal, mes: mesenteric, com: combined counts; n = 3–19 mice, one-way ANOVA, counts are plotted per single lymph node.

(D) Lymph node counts after treatment with FTY720 (egress block) or integrin-blocking antibodies (homing block); n = 3–5 mice, one-way ANOVA with Tukey’s multiple comparisons test.

(E) Lymphocyte subpopulations after homing block (left) and egress block (right); n = 3 mice. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗∗p < 0.0001. All data are represented as mean ± SEM. See also Figure S1.