Figure 3.

Oscillations of Circadian Clock Genes in Lymph Nodes Control Cellularity

(A) Q-PCR analysis of circadian clock genes in LN over 24 hr; n = 3–5 mice, one-way ANOVA.

(B) Circadian clock gene mRNA profiles in sorted CD4⁺ T cells from Bmal1^flox/floxxCd4-cre and control animals; n = 3–10 mice, two-way ANOVA.

(C) Lymph node CD4 and CD8 T cell counts in control and T cell specific Bmal1^−/− mice, n = 3–9 mice, one-way and two-way ANOVA.

(D) LN homing of lymphocytes harvested from control or T cell-specific Bmal1^−/− mice at ZT5 or ZT13 into WT hosts; n = 10–34 mice, one-way ANOVA with Tukey’s multiple comparisons test.

(E) CCR7 surface expression on T lymphocyte subpopulations in LN of control and T cell-specific Bmal1^−/− mice; n = 3–5 mice, one-way ANOVA.

(F) Q-PCR analysis of CD4⁺ T cell Ccr7 over 24 hr in control and T cell-specific Bmal1^−/− mice; n = 4–8 mice, one-way and two-way ANOVA. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗∗p < 0.0001. All data are represented as mean ± SEM. See also Figure S2.