Skip to main content
. 2016 Sep 14;21(2):208–221. doi: 10.1111/jcmm.12957

Figure 4.

Figure 4

Alternol inhibits the constitutive activation of STAT3 and the expression of its gene products in 143B and MG63 cells (A and B) 143B and MG63 cells were treated with 5 μM of alternol for 12 hrs or followed by 25 ng/ml of IL‐6 for 30 min. After treatment, the localization of phosph‐STAT3 was analysed by immunofluorescence. STAT3 and phosph‐STAT3 protein expression were measured by immunoblotting (C). (D) 143B and MG63 cells were transfected with pGMSTAT3‐Lu for 24 hrs and were then incubated with vehicle control or indicated alternol for 12 hrs and STAT3 activity was measured using the STAT3‐dependent dual luciferase assay kit. (E and F) 143B and MG63 cells were treated with vehicle or indicated alternol for 12 hrs, the expression of STAT3, phosph‐STAT3, Src, phosph‐Src, cyclin D1, Bcl‐xl and survivin were detected by immunoblotting. *P < 0.05 versus with vehicle control, **P < 0.01 versus with vehicle control.