Figure 1.

Solamargine inhibited growth in multiple lung cancer cell lines and induced cell growth arrest in H1299 cells. (A) Lung cancer cell lines (H1650, H1975, PC9, A549 and H1299) were treated with solamargine (6 μM) for up to 48 hrs. Afterwards, the cell viability was determined using the MTT assay as described in the Materials and methods section. (B) H1299 cells were treated with increased concentrations of solamargine for up to 48 hrs. Afterwards, the cells were collected and processed for analysis of cell cycle distribution by flow cytometry after propidium iodide (PI) staining. And the percentages of the cell population in each phase (G0/G1, S and G2/M) of cell cycle were assessed by Multicycle AV DNA Analysis Software. Data are expressed as a percentage of total cells. Values are given as the mean ± SD from three independent experiments performed in triplicate. *Significant difference as compared with the untreated control group (P < 0.05).