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. 2016 Sep 13;21(2):222–233. doi: 10.1111/jcmm.12958

Figure 5.

Figure 5

Overexpression of EP4 blocked the solamargine‐inhibited c‐Jun protein expression; exogenously expressed c‐Jun feedback reversed solamargine‐induced phosphorylation of ERK1/2. (A) A549 and H1299 cells were transfected with control and EP4 expression vectors for 24 hrs before exposing the cells to solamargine (6 μM) for an additional 24 hrs. Afterwards, the EP4 and c‐Jun proteins were determined using Western blot. GAPDH was used as internal control. (B–C) A549 and H1299 cells were transfected with the control or expression construct of c‐Jun for 24 hrs before exposing the cells to solamargine (6 μM) for an additional 24 hrs. Afterwards, the expressions of c‐Jun (B–C), EP4 (C) and p‐ERK1/2 (B) were determined by Western blot. GAPDH was used as internal control. Values in bar graphs were given as the mean ± SD from three independent experiments. *Significant difference compared with the untreated control group (P < 0.05). **Significant difference from solamargine treated alone (P < 0.01). ERK1/2, extracellular signal‐regulated kinases 1/2.