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. 2017 Jan 25;7:41221. doi: 10.1038/srep41221

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A_1–3) Images of the ipsilateral side of a section cut from the L4-L5 spinal segments. p-S10H3-expressing structures (arrows) 5 minutes after burn injury also express the neuronal nucleus marker NeuN. (B_1–3) Images of the ipsilateral side of a section cut from the L4-L5 spinal segments. Microglia identified with an anti-ionised calcium-binding adaptor protein 1 (IBA1) antibody do not express p-S10H3 after burn injury. (C_1–3) Images of the ipsilateral side of a section cut from the L4-L5 spinal segments. Astrocytes identified with an anti-glial fibrillary acidic protein (GFAP) antibody do not express p-S10H3 after burn injury. (Scale bar, 200 μm; scale bar in inset, 100 μm.) Dotted and dashed lines indicate the surface of the spinal cord and the white-grey matter border on each image.