Table 2.
Bone parameters evaluated ex vivo by µ-CT at proximal tibial epiphysis and metaphysis
| Parameter | Normal rats | MMT surgery | MMT plus amputation | Sham plus amputation |
|---|---|---|---|---|
| Proximal tibial epiphysis (cortical and cancellous bone) | ||||
| TV, mm3 | 1.65±0.11 | 1.87±0.14 | 1.83±0.07 | 1.67±0.11 |
| BV, mm3 | 0.76±0.10 | 0.74±0.09 | 0.67±0.04†,‡ | 0.64±0.05†,‡ |
| BV/TV, ratio | 0.46±0.03 | 0.43±0.02 | 0.37±0.03††,‡ | 0.38±0.02††,‡ |
| BMD, g/cm2 | 985.14±16.44 | 990.85±14.89 | 983.37±14.97 | 1005.05±11.05 |
| Proximal tibial metaphysis (cortical and cancellous bone) | ||||
| TV, mm3 | 2.81±0.05 | 2.88±0.04 | 2.87±0.05 | 2.80±0.14 |
| BV, mm3 | 0.73±0.06 | 0.57±0.06††† | 0.24±0.07†††,‡‡‡ | 0.26±0.05†††,‡‡‡ |
| BV/TV, ratio | 0.26±0.02 | 0.20±0.02††† | 0.08±0.02†††,‡‡‡ | 0.09±0.02†††,‡‡‡ |
| BMD, g/cm2 | 929.74±32.06 | 922.53±57.59 | 848.15±40.35 | 943.72±68.42 |
| Proximal tibial metaphysis (cancellous bone only) | ||||
| TV, mm3 | 0.93±0.01 | 0.95±0.01 | 0.94±0.02 | 0.96±0.00 |
| BV, mm3 | 0.15±0.02 | 0.07±0.01††† | 0.01±0.01†††,‡‡‡ | 0.03±0.02†††,‡‡‡ |
| BV/TV, ratio | 0.16±0.02 | 0.08±0.01††† | 0.01±0.01†††,‡‡‡ | 0.03±0.02†††,‡‡‡ |
| TbN, 1/mm | 2.82±0.35 | 1.65±0.26††† | 0.32±0.19†††,‡‡‡ | 0.63±0.32†††,‡‡‡ |
| TbT, ìm | 0.056±0.002 | 0.048±0.005† | 0.039±0.007†††,‡‡‡ | 0.041±0.010†,‡ |
| TbS, ìm | 0.30±0.04 | 0.57±0.09††† | 4.94±4.64†††,‡‡‡ | 2.01±1.23†††,‡‡‡ |
| BMD, g/cm2 | 918.43±19.86 | 923.92±37.10 | 864.96±41.79 | 921.60±45.66 |
†p<0.05 versus normal; ‡p<0.05 versus MMT; ††p<0.01 versus normal; ‡‡p<0.01 versus MMT; †††p<0.001 versus normal and ‡‡‡p<0.001 versus MMT.
BMD, bone mineral density; BV, bone volume; MMT, medial meniscal tear; TbN, trabecular number; TbS, trabecular separation; TbT, trabecular thickness; TV, tissue volume; μCT, micro-CT.