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. 2017 Jan 15;13(1):110–121. doi: 10.7150/ijbs.16312

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© Ivyspring International Publisher. This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions.

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Effects of knockdown of Rictor expression by shRNA in mES cells on the cardiomyocyte differentiation. (A) Knockdown of Rictor (shRNA-Rictor) in mES cells during the differentiation. (B) The beating phenotype evaluated from day 5+1 to day 5+3 after EBs plating. (C) The expression levels of blastodermic layer proteins in the early cardiomyocyte differentiation. (D) The expression of Nkx2.5 in cryosections of EBs on day 5 by immunofluoresence analysis. (E) The expression level of sarcomeric protein detected by western blot. (F) Quantification of sarcomeric protein (α-Actinin) by flow cytometry on day 5+3. (G) Immunofluorescence analysis of the expression and distribution of sarcomeric α-Actinin (red) on day 5+3. Nuclei were stained with DAPI (blue). Bar=10 μm or 100 μm. (H) The proliferation rate of mES cells after infection by shRNA-Rictor. (I) The proportion of α-Actinin-positive cells after purification. Data were represented as means ± SD of three independent experiments. *P<0.05, **P<0.01 (shRNA-Rictor vs shRNA-Con).