Fig. 6.

Inhibition of Stat3 activity ameliorates the changes in levels of mTOR components observed in VDR^−/− soleus muscles. a–c (i) and (ii) Representative western blot analysis of lysates from b were probed with p-Smad3 (a), p-p70S6K (b), and p-rpS6 (c) antibodies. The p-Smad3 blot was also probed with MHC (slow) as a representative example to mark the identity of the lanes. a–c, (iii) Graphs show fold differences in p-Smad3, p-p70S6K, and p-rpS6, respectively, that were calculated by normalizing intensities with respective GAPDH values (n = 5 mice/group, N = 3 independent experiments) (*p < 0.05, **p < 0.01). While p-Smad3 levels were reduced in the soleus muscles of inhibitor-treated V mice, p-p70S6K and p-rpS6 levels were increased