Fig. 7.

Different levels of Stat3 expression and activity in VDR^−/− muscle subsets amenable to pharmacological inhibition could be linked to preferential fiber type expression of p-Stat5 and its dependence on vitamin D signaling. a Representative western blot analysis of lysates from the soleus and TA muscles isolated from 6-week-old WT and V mice were probed with p-Stat5 and total Stat5 antibodies. Graph below show the fold differences in levels of p-Stat5 to total Stat5 protein that was calculated after normalizing band intensities with respective GAPDH values (n = 5 mice/group, ***p < 0.005). Levels of p-Stat5 are significantly higher in the TA muscles than those in the soleus muscles and are downregulated in the absence of VDR signaling. b Western blot analysis of lysates from the soleus and TA muscles of V mice were probed with p-Stat3 and total Stat3 antibodies. Graph below shows the fold differences in levels of p-Stat3 and total Stat3 after normalizing band intensities with respective GAPDH values (n = 5, ***p < 0.005, **p < 0.01). Blots were probed with MHC (slow) to mark the identity of the lanes. The TA muscles express significantly higher levels of p-Stat3 and total Stat3 compared to the soleus muscles from V mice