Figure 5. M1-C clustering at the GUV surface.

Fluorescence projections of the protein signal on (a) TBE-GUVs and (b) DOPS-GUVs vesicles. For TBE-GUVs, proteins assemble into a network-like structure on the vesicle surface. For DOPS-GUVs the protein forms clusters but does not assemble into networks. A quantitative estimate of the number of clusters and network density can be found in Supplementary Fig. S4 (c) Z-stack images of a TBE-GUV vesicle incubated with 345 nM M1-C taken at the bottom (glass side) at the equator and at the top of the GUV. Scale bars: 10 μm.