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. 2017 Jan 25;7:40801. doi: 10.1038/srep40801

Figure 6. Colocalization experiment using labeled lipids.

Figure 6

(a) Projections of TBE-GUVs with a negatively charged fluorescently-labeled lipid (Bodipy TMR PI(4,5)P2). The red signal corresponds to Bodipy TMR PI(4,5)P2 incorporated to the GUV membrane and the green signal to Alexa-488 M1-C. The negatively charged fluorescent lipids co-localize with the protein network. Scale bar: 10 μm. (b) (i) Projections of TBE-GUVs containing small amounts of bodipy-ceramide, a lipid that does not interact with M1 C. The red signal corresponds to bodipy-ceramide lipids incorporated to the GUV membrane and the green signal to Alexa-488 M1-C. In both cases, the protein bulk concentration was 1.4 μM. (ii) Control experiment using vesicles without fluorescent lipids at maximum laser power: only signal noise is detected, showing the absence of bleed-through between the fluorescence channels. Scale bar: 10 μm.