Fig. 5.

Colocalization of Nef, TNF and Notch1 in lymph node tissue. (a) Costaining of Nef, TNF and CD45 in healthy tonsil tissue and a lymph node obtained from a non-viremic HIV-infected individual. The white arrow (bottom left panel) depicts the speckled TNF staining pattern suggesting vesicular TNF secretion. (b) MELC analysis of the HIV lymph node and healthy tonsil reveal different staining patterns and expression levels of antigens. All panels show the same section of tonsil or lymph node tissue, stained with nine different antibodies and Propidiumiodid (PI). (c, d) TNF, Notch1 and Nef colocalize in cells with budding membrane structures (white arrows in (d)).