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H19loss of function reduces CRC aggressiveness. (a, b)H19 effect on proliferation, determined with cell counting kit 8 assay. (c, d)H19 effect on DNA synthesis in CRC cells, determined with Click-iT EdU assay. (e, f)H19 effect on cell migration, determined with cell scratch assay. Typical images on left, and quantifications on right. Difference was determined using 2-way ANOVA with GraphPad software. * P < 0.05. See also Fig. S2.
