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. 2017 Jan 25;48:3. doi: 10.1186/s13567-016-0407-0

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Brightfield image of a zebrafish embryo inoculated with a fluorescent E. coli strain. A Brightfield image indicating the inoculation site of E. coli K46/pGEN-GFP (LVA) in the circulatory valley (arrow). B At 12 hpi, the bacteria are located throughout the vasculature of the embryo, but with minimal intrusion into the surrounding tissues.