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. 2017 Jan 25;8:40. doi: 10.3389/fmicb.2017.00040

Table 1.

Geochemical data for microcosms over the 20-day study.

Variable Live Killed
Day 0 Day 5 Day 11 Day 15 Day 20 Day 0 Day 20
Average SDa Average SD Average SD Average SD Average SD Average SD Average SD
pH 7.9 0.0 8.1 0.2 8.1 0.2 8.1 0.4 NAb NA 7.9 0.0 NA
Fe(II) (mM) 8.0 2.4 9.0 0.03 9.8 0.03 9.1 0.01 15.0 2.2 4.5 0.4 NA
Ammonium (μM) 178 1.6 153 12 143 23 154 17 137 3.3 202 6.1 212 18
Chloride (mM) 3.34 0.13 3.27 0.09 3.29 0.12 3.50 0.21 3.29 0.23 3.36 0.12 3.38 0.23
Acetate (μM) 60 4.3 44 13 6.7 2.8 42 22 620c 35c 120 26 110 35
Propionate (μM) <DLd < DL <DL < DL <DL <DL <DL
Sulfate (mM) 8.67 0.11 8.36 0.46 8.26 0.59 8.66 0.90 8.10 0.86 9.00 0.31 8.75 0.39
Nitrate (μM) 114 5.6 118 12 111 14 98 41 120 25 115 2.5 109 17
Nitrite (μM) <DL <DL <DL <DL <DL <DL <DL
DOC (μmol) 17.7 14.4 9.1 7.0 5.9 2.2 5.9 3.1 2.5 0.6 69.3 10.5 57.5 7.9
DOC δ13C (%0) −28.54 < DL <DL <DL <DL −23.82 0.44 −23.56 0.19
Nitrous oxide (ppm) < DL < DL < DL <DL <DL <DL <DL
Methane (ppm) 1.3 0.0 4.3 0.29 6.4 0.19 7.7 0.76 15.4 0.56 1.3 0.0 1.3 0.0
Hydrogen (ppm) <DL <DL <DL <DL <DL <DL <DL
a

Standard deviation.

b

Not analyzed.

c

Note that the acetate data on day 20 (live) represents the average of 3 replicates that were attached to the respirometer; a fourth sample, not attached to the respirometer, did not show an increase in acetate. If all replicates were included, acetate would be 470 ± 310 μM.

d

Below detection limit: propionate, 10 μM; nitrite, 10 μM; DOC δ13C, 0.9 μmol C; nitrous oxide, 0.2 ppm; hydrogen, 40 ppm.