Figure 6. Kinetic behavior of MetaChi18A. A, Colloidal chitin as substrate; B, 4-MUF-(GlcNAc)2 as substrate. Colloidal chitin (g/L) and V0 (nmol of reducing sugar/min); 4-MUF-(GlcNAc)2 (µM) and V0 (nmol of MUF/min). Reactions were performed at 25 mM sodium acetate buffer, pH 5, at 50°C (A) or 37°C (B). The black line is the model that better fits the data: (A) allosteric kinetic+substrate inhibition, and (B) substrate inhibition; Blue dotted line: allosteric kinetic; Orange slashed line: Michaelis-Menten kinetic.
