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. 2017 Jan 25;7:41392. doi: 10.1038/srep41392

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Calibration (a–c) curves of the simple-simple, simple-MGB and simple-LNA dual-probe assays in opposite orientations. The curves were prepared for a dilution gradient from 1e2 to 1e7 NA standard copies per μl of template in triplicates. The dual-probe assays are highlighted in red and the corresponding single-probe counterparts in probe-specific colours. The probe sequences and their schematic representation and colouring are shown in Figs 1 and 2. The ΔCq curves (d–f), representing the Cq differences between the dual-probe versus the corresponding A and B assays at each concentration point, were coloured in green and grey, respectively. For the data regarding additional dual-probe assays, either in opposite or tandem orientations, please refer to Supplementary Information 1, Figure S1.2 and Supplementary Information 2, Figure S2.2, respectively.