Figure 6. The effect of trastuzumab on CASP3, CCNE2, and APAF1 protein expression by western blot analysis.

(A) Levels of APAF1, CCNE2, and CASP3 proteins were measured in BT474 wt, BT474r, and HCC1954 cells exposed to trastuzumab (15 μg/ml for 7 days). Actin was used as loading control. (B) Levels of CCNE2 protein in BT474 wt, BT474r, and HCC1954 cells transfected with miR-30b mimetic.