Table 6. STD-NMR binding analysis of compounds with M. avium carveol dehydrogenase MyavA.01326.l.
| Compound | Description | Spike molecule | %STD (Day 0) | %STD cofactor spike (Day 0) | %STD cofactor spike (Day 7) |
|---|---|---|---|---|---|
| NAD | Cofactor | N/Aa | 3.6 | N/A | N/A |
| NADH | Cofactor | N/A | 2.1 | N/A | N/A |
| (−)-carveol | Substrate | NAD | 10.2 | 3.9 | 8.8 |
| (R)-(−)-carvone | Product | NADH | 13.5 | 14.0 | 14.4 |
| (S)-(+)-carvone | Product | NADH | 20.5 | 21.3 | 19.7 |
| Tricyclazol | Inhibitor | NAD | 17.5 | 19.9 | 16.1 |
| Tricyclazol | Inhibitor | NADH | 19.0 | 19.5 | 17.3 |
| N,N-dimethyl-4-nitosoanaline | Redox partner | NADH | 7.5 | 8.3b | 14.9b |
| 2,6-dichloroindophenol (DCIP) | Redox partner | NAD | 11.4 | 11.5 | 13.8 |
| 2,6-dichloroindophenol (DCIP) | Redox partner | NADH | 10.2 | 3.0b | 13.0b |
aN/A = not applicable.
bPartial conversion of NADH to NAD was observed via changes in chemical shift in these samples. No change in chemical shift was observed for any of the other compounds in these samples.