Fig. 5.

Expression of miR-9 increased with cell density in cultured 9 L glioma cells. a miR-9 expression was significantly higher in 9 L cells plated at 50000 or 250,000 cells/cm² compared to cells plated at 5000 cells/cm². **p < 0.01 vs. 5000 cells/cm² control. b Inhibition of gap junctions with carbenoxolone did not decrease miR-9 expression in 9 L cells plated at 50000 or 250,000 cells/cm² compared to untreated control. *p < 0.01 vs. untreated control. C, Effect of glucose deprivation on miR-9 expression in 9 L cells plated at 5000, 50,000, or 250,000 cells/cm² compared to cells cultured in normal glucose conditions. *p < 0.01 vs. normal glucose control