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. 2017 Jan 17;11:225–239. doi: 10.2147/DDDT.S123919

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© 2017 Wu et al. This work is published and licensed by Dove Medical Press Limited

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Fluorescence spectra of HMCEF.

Notes: (A) Fluorescence spectra of HMCEF in PBS buffer (concentration: 0.5 μM, pH =7.4, λ_ex =254 nm) explain the fluorescence quenching induced by 10 μL of CT DNA (final concentrations: 0, 8, 16, 24, 32, 40, 48, 56, 64 and 72 μM) in PBS. (B) Fluorescence spectrum of EB plus CT DNA and HMCEF (the concentration of EB is 5 μM, the concentration of DNA is 10 μM and the concentrations of HMCEF are 0, 10, 20, 30, 40, 50, 60 and 70 μM, respectively).

Abbreviations: CT DNA, calf thymus DNA; EB, ethidium bromide; HMCEF, N-(3-hydroxymethyl-β-carboline-1-yl-ethyl-2-yl)-l-Phe; PBS, phosphate-buffered saline.