Figure 7.

Effect of atorvastatin on the activities of the binding of nuclear transcription factors CREB proteins and CRE gene. INS-1 cells were pretreated with 50 μM atorvastatin or manumycin A, respectively, and subjected to high glucose (25 mM) for 6 hours. The effect of atorvastatin on the binding of nuclear transcription factor p-CREB and cAMP response element in INS-1cells was examined via chromatin immunoprecipitation assay–polymerase chain reaction. CREB = cAMP response element-binding protein.