Fig. 4. CD4⁺CD25⁺FOXP3⁺Tregs of total lymphocytes in spleens. Treg cells count in the spleen was measured by flow cytometry. (A) The analysis of CD4 vs FOXP3 expression was gated in CD4⁺CD25⁺ cells. (B) CD4⁺CD25⁺FOXP3⁺Tregs were calculated in each group (n=5 for each group; ^*P<0.01).