Table 2.

Correlation of Apparent Mode-shift with Deactivation Kinetics

	Activation GV			Deactivation GV			Mode-shift		Deactivation τ—τf (ms)
	Vh (mV)	k (mV)	n	Vh (mV)	k (mV)	n	Vh (mV)	n	−110 mV	n
WT	−22.8 ± 1.1	10.8 ± 0.7	13	−54.2 ± 1.6	7.8 ± 0.4	13	−31.4 ± 2.1	13	94.8 ± 9.3	8
R4D	−14.9 ± 1.0	9.2 ± 0.3	5	−22.8 ± 1.1	7.5 ± 0.8	5	−7.8 ± 1.6	5	27.5 ± 2.5	4
R5E	−17.6 ± 2.0	9.6 ± 0.3	5	−30.1 ± 1.4	7.8 ± 0.5	5	−12.5 ± 1.0	5	47.9 ± 5.7	4
D456A	−1.0 ± 1.2	7.5 ± 0.4	5	−7.0 ± 1.0	7.5 ± 0.6	5	−6.0 ± 0.9	5	35.8 ± 2.5	5
E480R	−2.8 ± 0.6	10.0 ± 0.2	5	−14.0 ± 1.7	8.4 ± 0.5	5	−11.8 ± 2.3	5	47.0 ± 3.5	5
D509A	14.3 ± 1.5	11.0 ± 0.2	4	8.3 ± 0.7	9.9 ± 0.7	4	−6.0 ± 1.4	4	22.0 ± 2.0	4
R537K	−8.6 ± 3.7	8.0 ± 0.3	6	−9.8 ± 3.7	8.3 ± 0.4	6	−1.2 ± 0.7	6	55.2 ± 2.3	5
R541G	−35.2 ± 1.5	13.2 ± 0.5	5	−41.7 ± 1.2	11.7 ± 0.4	5	−6.5 ± 0.6	5	42.8 ± 1.6	7
Y542G	−4.9 ± 2.1	14.8 ± 1.0	5	−14.9 ± 1.4	11.1 ± 0.7	5	−10.0 ± 1.6	5	12.9 ± 0.5	9
E544G	−22.4 ± 0.5	8.5 ± 0.1	7	−25.3 ± 0.8	7.5 ± 0.2	7	−2.9 ± 0.6	7	18.9 ± 0.6	5

Activation GV relations were collected from oocytes held at −80 mV and subjected to 2 s depolarizing steps to +50 mV in 10 mV increments followed by a 2 s step to −110 mV. Deactivation GV relations were collected from oocytes held at −80 mV and subjected to a 500 ms depolarizing step +60 mV followed by 8 s steps from –110 mV to +40 mV in 10 mV increments followed by a repolarizing step to −110 mV. Boltzmann fits were used to obtain the V_h and k values. Deactivation τ was obtained by fitting the deactivation tail current decay with a double exponential function. The value τ_fast is reported, because current decay at −110 mV was dominated (>85%) by the fast component.