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Representative micrographs of TOP2A FISH. (A) One tumor with DM-type amplification typically displayed multiple individual TOP2A signals (orange). (B) One tumor with HSR-type amplification showed clustered TOP2A signals, resistant to RNase pre-treatment. (C) A TOP2A-deleted tumor demonstrated absence or 1 copy of TOP2A signals and 2–4 CEP 17 signals (green). (1000x magnification).
