Table 9.
Glucose and lipid profiles of blood serum from control, simvastatin, Sim/Ram, ramipril, and CR-treated mice
| BW | TC | HDL | LDL | TG | NEFA | Glu | |
|---|---|---|---|---|---|---|---|
| (g) | (mg/dL) | (mg/dL) | (mg/dL) | (mg/dL) | (mEq/L) | (mg/dL) | |
| Control | 41.9 ± 3.2b | 183.1 ± 40.0 | 175.5 ± 36.9 | 22.4 ± 8.0 | 40.8 ± 13.2 | 0.905 ± 0.157 | 128.4 ± 13.6 |
| Simvastatin | 42.8 ± 2.2 | 162.9 ± 32.5 | 162.4 ± 21.7 | 18.7 ± 7.9 | 42.5 ± 15.4 | 1.032 ± 0.231 | 146.2 ± 40.4 |
| Sim P valuea | 0.609 | 0.404 | 0.518 | 0.481 | 0.853 | 0.342 | 0.403 |
| Sim/Ram | 39.9 ± 5.0 | 168.2 ± 25.3 | 166.9 ± 14.7 | 20.4 ± 9.7 | 80.9 ± 36.1 | 0.901 ± 0.269 | 241.4 ± 21.6 |
| Sim/Ram P value | 0.489 | 0.500 | 0.648 | 0.726 | 0.067 | 0.980 | 0.000 |
| Ramipril | 44.16 ± 1.8 | 231.7 ± 24.1 | 220.7 ± 23.4 | 33.9 ± 8.7 | 70.7 ± 11.1 | 1.051 ± 0.235 | 130.2 ± 55.4 |
| Ramipril P value | 0.210 | 0.060 | 0.060 | 0.065 | 0.006 | 0.292 | 0.947 |
| 40 % CR | 33.9 ± 2.7 | 159.3 ± 7.4 | 141.0 ± 28.7 | 9.7 ± 2.6 | 130.8 ± 58.2 | 0.729 ± 0.106 | 122.0 ± 10.6 |
| 40 % CR P value | 0.004 | 0.257 | 0.143 | 0.027 | 0.028 | 0.076 | 0.432 |
Five male, B6C3F1 mice in each treatment group were 448 days of age at the time they were bled by cardiac puncture. The treated mice received each drug or combination of drugs in their food for 99 days. The 40 % CR group was shifted to 11 kcal/day/mouse of AIN-93 M 20 % Restricted Diet (Diet no. F06298, Bioserv) for 2 weeks, and thereafter to 7.46 kcal/day of AIN-93 M 40 % restricted diet (diet number F05314, Bio-Serv). The stepped reduction in calories reduces stress-induced mortality during the period of initial weight loss. The total time on a CR diet was 99 days. The control mice were fed control diet for the same period of time. Procedures and husbandry were as described for the other mice in this report. Glucose measurements were made using the FreeStyle Lite Blood Glucose Monitoring System (Abbot Diabetes Care, Alameda, CA). Other blood tests were performed by the Comparative Pathology Laboratory, University of California, Davis
BW body weight, CR calorically restricted, Glu glucose, HDL high-density lipoprotein, LDL calculated low density lipoprotein, NEFA nonesterified fatty acid, TC total cholesterol, TG triglycerides
a P values were determined using a two-tailed t test. Each P value is applicable to the measurements immediately above it
bMean ± standard deviation. Values indicative of significance are shown in bold for convenience