Table 1. Quality of the tested cryopreservation protocols.

	Pre-cryoViability	Post-cryoViability	RecoveryYield	Ability togenerate NS
Protocol-1	N/D	76,90 ± 2,4%	71,44 ± 7,2%	+++
Protocol-2	90,51 ± 0,7%	66,47 ± 12%	59,32 ± 14,4%	-
Protocol-3	91,65 ± 1,8%	75,43 ± 5,8%	34,36 ± 4,5%	+
Protocol-4	89,14 ± 1,8%	92,23 ± 7,3%	29,60 ± 5,4%	-

Cell survival was determined by tripan blue staining for each cryovial (n = 5). Data represented as mean ± SEM. After cell plating to form NS, the cultures were classified as: (-) not able to form NS; (+) slow formation of primary NS; (+++) fast and vigorous formation of primary and secondary NS. N/D; Not determined.