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. 2017 Jan 12;13(1):e1006157. doi: 10.1371/journal.ppat.1006157

Fig 4. The E409V mutation affects the self-association of OsDRP1E.

Fig 4

(A) Yeast two-hybrid assays using the HIS3 reporter to detect the self-interaction of OsDRP1E. Yeast cells transformed with bait and prey constructs as indicated were sequentially diluted 10-fold and plated on synthetic dextrose (SD) medium without Trp, Leu and His amino acids (SD-LTH) and with 0 mM or 40 mM 3-amino-1,2,4,-triazole (3AT), respectively. Yeast cells that either grew in the presence of 40 mM 3AT or were stained blue by X-gal indicate an interaction. (B) Immunoblot detection of GFP-tagged OsDRP1E and E409V expressed in N. benthamiana using Blue Native-PAGE (upper panel) and SDS-PAGE (bottom panel). Blue Native-PAGE followed by immunoblot analysis was used to detect the oligomerization of OsDRP1E-GFP and E409V-GFP. SDS-PAGE followed by immunoblot analysis was used to detect the expression levels of OsDRP1E-GFP and E409V-GFP.