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HSP90 and VDAC1 served as the loading control for cytosolic and mitochondrial protein, respectively. Y: Young leaves from four-week-old plants. O: Old leaves from eight-week-old plants. Numbers below the band in first panel represent the relative cytochrome c levels in cytosol and mitochondria as compared to HSP90 and VDAC1, respectively, using the Image J software.
