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. 2017 Jan 15;33(1):55–62. doi: 10.5487/TR.2017.33.1.055

Fig. 5.

Fig. 5

Effect of Oenothera laciniata methanol extract on mRNA expression of tyrosinase, TRP-1, TRP-2, and MITF-M in melan-a cells. mRNA expression in the cells treated with the test materials for 48 hr was analyzed using RT-PCR. (A) A representative gel (upper) and semi-quantification of tyrosinase mRNA expression by normalization of β-actin (lower). (B) A representative gel (upper) and semi-quantification of TRP-1 mRNA expression by normalization of β-actin (lower). (C) A representative gel (upper) and semi-quantification of TRP-2 mRNA expression by normalization of β-actin (lower). (D) A representative gel (upper) and semi-quantification of MITF-M mRNA expression by normalization of β-actin (lower). C: control, PC: arbutin, OLME: Oenothera laciniata methanol extract. Values are the mean ± SD of three independent experiments. Statistically significant differences (p < 0.05) identified using an ANOVA and Duncan’s multiple range test are indicated by different letters (a > b > c > d).