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. 2016 Jun 13;49(1):230–245. doi: 10.4143/crt.2015.506

Fig. 10.

Fig. 10.

dsRNA and siRNA targeted autophagy–related gene LC3 (siLC3) inhibited angiogenesis in vitro and in vivo. (A) Effects of dsRNA and siLC3 on human umbilical vein endothelial cell (HUVEC) tube-formation (×100). Both dsRNA and siLC3 inhibited the tube formation of HUVECs on matrigel; however, an obvious organized network of tubes was observed in untreated HUVECs. (B) The tumor blood flow changes in B-model ultrasound diagnosis. Compared to the phosphate buffered saline control group with obvious blood flow in tumor and basilar part, blood flow was moderately inhibited in dsRNA and siLC3 alone, while most significantly reduced in siLC3+dsRNA (the red area for flow toward the probe, and the blue area for flow back to the probe). (C) CD34 expressing positive microvessels in immunohistochemistry staining (×400). The results of CD34 expressing microevessels by immunohistochemistry staining were similar to that in HUVEC tube–formation or B-model ultrasound diagnosis.