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Apoptosis in HepG2 cells detected by flow cytometry (A), Hoechst33285 (B), and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) staining (C) (arrows indicating apoptotic nuclei were bright blue in Hoechst33285 staining, and bright green in TUNEL staining, ×200). PI, propidium iodide; siLC3, siRNA targeted autophagy–related gene LC3.
