Fig. 1.

Expression of Escherichia coli cytosine deaminase (CD) and human interferon β (IFN-β) genes in genetically engineered human neural stem cells. Expression of introduced therapeutic genes, CD and IFN-β, was confirmed. After total RNA extraction from HB1.F3.CD and HB1.F3.CD.IFN-β cells, cDNAs were synthesized. These cDNAs were amplified by polymerase chain reaction (PCR), and PCR products were confirmed by gel electrophoresis. The transcripts of E. coli CD (559 bp) and human IFN-β (296 bp) were detected by PCR. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH, 351 bp) was used as an internal control.