Fig. 2.

In vitro antiproliferative effect of genetically engineered human neural stem cells (hNSCs) with 5-fluorocytosine (5-FC). Since SW-620 cells (4×10³ cells/well) were seeded in 96-well plates, after 24 hours, hNSCs were seeded in the same well and treated with 5-FC for 3 days. Cell viability was measured by 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay. (A) Cytotoxic effect of various concentrations of 5-FC or 5-fluorouracil (5-FU) on SW-620 cells. (B) Effect of hNSCs with 5-FC (100, 200, 300, 400, and 500 μg/mL) on the SW-620 co-culture system. (C) Microscopic analysis of cells (×200) after 3 days following treatment with hNSCs in the presence of 5-FC (500 μg/mL). Phosphate buffered saline (PBS) treatment was used as a negative control. Data are represented as mean±standard error of the mean. ^*p < 0.05 vs. control.