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. 2016 May 3;49(1):79–91. doi: 10.4143/crt.2015.503

Fig. 3.

Fig. 3.

Migratory capacity of human neural stem cells (hNSCs) towards SW-620 in vitro. (A) Human fibroblasts (hFB) and SW-620 cells (1×105 cells/well) were seeded on the lower wells of 24-well plates. hNSCs (1×105 cells/well) were stained with CM-Dil and seeded in the fibronectin precoated upper insert chambers. DAPI staining solution was added to lower wells for observation of SW-620 and human fibroblasts. The blue stained cells indicated SW-620 or hFB as a control. Red stained cells indicated migration of hNSCs from the upper insert chamber toward SW-620 or hFB. (B) Migratory ratio of hNSC was measured using a Cell Sense Dimension system (Olympus, Tokyo, Japan). Data are represented as mean±standard error of the mean. *p < 0.05 vs. hFB. (C) Expression of diverse chemoattractant factors was shown in SW-620 cells. SDF-1a, stromal cell-derived factor 1a; uPAR, urokinase receptor; CCR2, C-C chemokine receptor type 2; uPA, urokinase-type plasminogen activator; GAPDH, glyceraldehydes-3-phosphate dehydrogenase.