FIG 4 .
The WT and pho4Δ strains trigger a different cellular response in the lungs. Single-cell suspensions of lung cells from mice infected with the WT and the pho4Δ mutant were analyzed at 10 days postinfection using flow cytometry. (A) Summary data showing the percentages of CD4+ T cells, CD8+ T cells, dendritic cells, and Ly6Chi inflammatory monocytes (Mo.). (B) Representative flow cytometry plot showing the gating for eosinophils (Siglec-F+) and neutrophils (Ly6G+) and summary histograms of their proportions. Data shown are mean percentages plus SDs (n = 5). Statistical analysis was performed using Student’s t test. Values that are significantly different are indicated by bars and asterisks as follows: *, P < 0.05; **, P < 0.01.